LS850 Microscope

The powerful, new LS850 Microscope is the latest generation of our fully automated three-channel flagship model and offers the latest advances in optics, cameras, throughput, and user flexibility. The LS850 improves the image quality, motion speed, illumination, and software flexibility over the previous LS720 model. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your automated microscopy experiments.

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  • Introduction

    The LS850 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a multi-day time-lapse, the LS850 offers a whole new world of walk-away automated microscopy!

    Features and Benefits

     Images, tiled images, Z-stacks, time-lapse series, and videos recorded directly to your computer
     Bright field and phase contrast transmitted illumination
     LED excitation and high performance optics provide diffraction-limited (theoretical maximum) resolution
     Multi-OS software allows set-up and control across any locations, including microplates, microfluidic chips, slides, dishes, flasks, and deck-top chambers and custom arrays
     High speed stage with single digit micron precision and accuracy
     Long range motorized Z for autofocus and Z-stacks down to 100 nm
     Compact and robust design enables use inside cell culture hoods, incubators, hypoxic chambers, and gloveboxes
     Detects blue, green and red fluorophores, including common probes such as Hoechst, DAPI, FITC, Fluo-4, GFP, Texas Red & mCherry GFP
     Open source Python control code available
     Objective compatibility with standard lenses and available with optional automated 4-objective turret

    Like all of microscopes, the LS850 utilizes a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 590 nm LED light sources. The 5-filter set is comprised of 3 excitation filters, 1 triple-band dichroic, and 1 triple-band emission filter. The LS850 use a 12 bit monochrome CMOS sensor.

    Click here for LS850 Microscope Product Brochure.

    Excitation and Emission Profiles of LS850 filters

    Description

    Specifications
    LED Excitation:405 nm, 488 nm, and 589 nm
    Fluorescence Filters:Blue: Excitation 370-410 nm, Emission 429-462 nm
    Green: Excitation 473-491 nm, Emission 502-561 nm
    Red: Excitation 580-598 nm, Emission 612-680 nm
    Transmitted Modes:Bright Field, Phase Contrast
    Objectives:1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil)
    Camera:High Sensitivity Monochrome CMOS BSI Sensor; 5 MP 12-Bit
    Image FormatsJPG, BMP, TIF, or PNG
    Image SizeAdjustable up to 2100 x 2100 pixels
    Video Rates:25 FPS (exposure limited)
    Automated XY Stage:120 mm x 80mm travel, 1-3 micron positional repeatability
    Throughput:96-well, 3 channel fluorescence, w/o autofocus – 6 minutes 30 seconds
    Motorized Z:14mm travel, 100 nm step, image-based autofocus, Z-stacks
    Control Software:LumaviewPro, multi operating system application
    Computer Requirements:Windows 10, 11; Core i7, 512GB SSD, 8GB
    RAM MacOS with M1; 512GB SSD, 8GB RAM
    Linux Debian Distributions:512GB SSD, 8GB RAM
    Power Requirements:80-264 V, 50-60 Hz 10W typical, 40W Max
    Integration:Python source under the MIT Open Source License
    Dimensions:39.8 cm W x 29.7 cm D x 29.7 cm H
    [15.6 in W x 11.6 in D x 11.6 in H]
    Weight:10 Kg/22lbs
    Operating Conditions:0°C – 42°C, 5% – 95% RH non-condensing
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