Oligos designed using next generation FANA technology can effectively and efficiently perform:
Efficient knock down or regulation of the target RNA.
Ability to bind to the target RNA (mRNA, miRNA or lncRNA) in a high sequence specific manner.
No toxicity.
Efficient delivery without an external source (e.g. without a transfection agent, formulation, conjugate or viral vector).
mRNA Knockdown
AUMsilence
Key features: Superior alternative for siRNA, shRNA and CRISPR. No delivery reagents required formulations or viral vectors required. No toxicity
AUMsilence self delivering oligos achieve highly efficient and potent antisense-based gene knockdown. No need to use toxic delivery reagents. Especially designed for animal studies.
Highly recommended as a replacement for siRNAs, shRNA, CRISPR or other oligo approaches.
We take pride in having the simplest protocol for RNA silencing studies. FANA oligos can be self delivered and do not need any delivery reagents, formulations or viral vectors (like AAV etc.) approaches. This significantly reduces toxicity associated with delivery reagents.
Protocol:
Recommended dose is between 3 – 30 mg/kg in mice. Administration routes: Depending upon your experimental purpose you may choose from Subcutaneous, Intrathecal, Oral or Intestinal.
miRNA Inhibition
AUMantagomir
Key features: High binding affinity. High specificity. No transfection reagents required. No toxicity
AUMantagomir self transfecting oligos serve as potent antagomirs by binding with miRNAs and prevent their hybridization with their target mRNAs.
AUMmirblocker self transfecting oligos serve as competitive blockers/decoys against miRNAs to inhibit their binding with target mRNA. AUMmirblocker oligos will bind with the targeted regions on the mRNA
and inhibit binding of miRNA with the mRNA.
AUMantagomir and AUMmirblocker are especially designed for animal studies.
Highly recommended as a replacement for siRNAs, shRNA, CRISPR or other oligo approaches.
We take pride in having the simplest protocol for RNA silencing studies. FANA oligos can be self delivered and do not need any delivery reagents, formulations or viral vectors (like AAV etc.) approaches. This significantly reduces toxicity associated with delivery reagents.
Protocol:
Recommended dose is between 5 – 50 mg/kg in mice. Administration routes: Depending upon your experimental purpose you may choose from Subcutaneous, Intrathecal, Oral or Intestinal.
IncRNA Knockdown
AUMInc
Key features: Superior knockdown. No transfection reagents required. No toxicity. High specificity
AUMlnc oligos achieve potent RNase H-mediated cleavage of the target long non-coding RNA. No need to use toxic delivery reagents. Especially designed for animal studies.
Highly recommended as a replacement for siRNAs, shRNA, CRISPR or other oligo approaches.
We take pride in having the simplest protocol for lncRNA knockdown studies. FANA oligos can be self delivered and do not need any delivery reagents, formulations or viral vectors (like AAV etc.) approaches. This significantly reduces toxicity associated with delivery reagents.
Protocol:
Recommended dose is between 3 – 30 mg/kg in mice. Administration routes: Depending upon your experimental purpose you may choose from Subcutaneous, Intrathecal, Oral or Intestinal.
Exon Skipping
AUMskip
Key features: No transfection reagents required. No toxicity. High specificity
AUMskip oligos can be used for exon skipping experiments.
We take pride in having the simplest protocol for exon skipping studies. No need to use toxic delivery reagents. Especially designed for animal studies.
Highly recommended as a replacement for conventional oligo approaches that cause toxicity.
We take pride in having the simplest protocol for exon skipping studies. FANA oligos can be self delivered and do not need any delivery reagents, formulations or viral vectors (like AAV etc.) approaches. This significantly reduces toxicity associated with delivery reagents.
Protocol:
Recommended dose is between 20 – 100 mg/kg in mice. Administration routes: Depending upon your experimental purpose you may choose from Subcutaneous, Intrathecal, Oral or Intestinal.
Depending upon the experiment different time points can be used to measure knock down or related effects for up to several days using a single dose (and weeks in some cases).
In certain cases (especially for very fast growing cells) if the knock down effect is reduced after a few days, simply add more FANA oligos to the cell culture.
FANA oligos can be fluorescently labeled (or with any desired label) to monitor cellular uptake or biodistribution.
Still want to use transfection reagents: If, for any reason, you still prefer to use transfection reagents the recommended working concentration is from 1nM to 25 nM (depending upon the cell type).
Shipping and Storage:
FANA oligos are shipped in lyophilized form. Upon arrival store them in -20°C.
When ready to use, mix FANA oligos with sterile water or your favorite buffer. It is recommended to make multiple aliquots to avoid multiple freez thaw cycles.