Oligos designed using next generation FANA technology can effectively and efficiently perform:
Efficient knock down or regulation of the target RNA.
Ability to bind to the target RNA (mRNA, miRNA or lncRNA) in a high sequence specific manner.
No toxicity.
Efficient delivery without an external source (e.g. without a transfection agent, formulation, conjugate or viral vector).
mRNA Knockdown
AUMsilence
Key features: Superior alternative for siRNA, shRNA and CRISPR. No delivery reagents required formulations or viral vectors required. No toxicity
AUMsilence self delivering oligos achieve highly efficient and potent antisense-based gene knockdown by cleaving the mRNA mediated through RNase H. With FANA oligos there is no need to use toxic delivery reagents or conjugates. They are especially designed for fish and amphibian model systems. AUMsilence will cause cleavage of the target. AUMsilence oligos can then recycle and be used to cleave other copies of the specific mRNA target. This leads to low dose requirement. This is a much efficient and superior approach than other conventional approaches that do not degrade the target mRNA instantly thus causing a high dose requirement and unknown biological effects.
FANA oligos have several additional advantages over conventional splice or translation blocking oligos:
(1) FANA oligos cleave the mRNA via RNase H thus the efficiency of the knockdown can be quantified. This is not possible with the conventional translation blocking oligos. This aspect have several biological applications that are not feasible with conventional oligos that can just prevent splicing or block translation.
(2) FANA oligos can be used to target both protein-coding and noncoding RNAs. They can target miRNA and lncRNAs.
(3) Because of the high recycle rate and better efficiency than conventional splice or translation blocking oligos, FANA oligos can work at significantly lower doses.
Highly recommended as a replacement for siRNAs, shRNA, CRISPR or other conventional oligo approaches.
We take pride in having the simplest protocol for RNA silencing studies. FANA oligos can be self delivered and do not need any delivery agents or special conjugates. This significantly reduces toxicity associated with delivery reagents or conjugates.
miRNA Inhibition
AUMantagomir
Key features: Superior alternative for shRNA, CRISPR and other conventional oligo approaches. No special conjugates required. No toxicity.
AUMantagomir self delivering oligos serve as potent antagomirs by binding with miRNAs and prevent their hybridization with their target mRNAs.
AUMmirblocker self delivering oligos serve as competitive blockers/decoys against miRNAs to inhibit their binding with target mRNA. AUMmirblocker oligos will bind with the targeted regions on the mRNA
and inhibit binding of miRNA with the mRNA.
AUMantagomir and AUMmirblocker are especially designed for fish and amphibian model systems.
Highly recommended as a replacement for shRNA, CRISPR or conventional splice or translation blocking oligos approaches.
We take pride in having the simplest protocol for RNA silencing studies. FANA oligos can be self delivered and do not need any delivery reagents, conjugates, formulations or viral vectors (like AAV etc.) approaches. This significantly reduces toxicity associated with delivery reagents.
IncRNA Knockdown
AUMInc
Key features: Superior alternative for siRNA, shRNA, CRISPR and other conventional splice or translation blocking oligo approaches. No special conjugates required or delivery vehicle required. No toxicity.
AUMlnc oligos achieve potent RNase H-mediated cleavage of the target long non-coding RNA. No need to use toxic delivery reagents. Especially designed for fish and amphibian model systems.
Highly recommended as a replacement for CRISPR and other conventional splice or translation blocking oligos approaches. Further, some of these approaches are unable to target lncRNA.
We take pride in having the simplest protocol for lncRNA knockdown studies. FANA oligos can be self delivered and do not need any delivery reagents or formulations or conjugates. This significantly reduces toxicity associated with delivery reagents.
Exon Skipping
AUMskip
Key features: No transfection reagents required. No toxicity. High specificity
AUMskip oligos can be used for exon skipping experiments.
We take pride in having the simplest protocol for exon skipping studies. No special conjugates or delivery vehicle required. This significantly reduces toxicity associated with delivery reagents.
Highly recommended as a replacement for CRISPR and other conventional splice or translation blocking oligo approaches.
Translation Blocking
AUMblock
Key features: Superior alternative for other conventional oligo approaches. No special conjugates or delivery vehicle required. No toxicity.
AUMblock oligos oligos can be used for to block translation experiments. This will not cause the degradation of the mRNA.
We take pride in having the simplest protocol for such studies. No special conjugates or delivery vehicles required. This significantly reduces toxicity associated with delivery reagents.
Highly recommended as a replacement for other conventional oligo approaches that cause toxicity.
Depending upon the experiment different time points can be used to measure knock down or related effects for up to several days using a single dose (and weeks in some cases).
In certain cases (especially for very fast growing cells) if the knock down effect is reduced after a few days, simply add more FANA oligos to the cell culture.
FANA oligos can be fluorescently labeled (or with any desired label) to monitor cellular uptake or biodistribution.
Still want to use transfection reagents: If, for any reason, you still prefer to use transfection reagents the recommended working concentration is from 1nM to 25 nM (depending upon the cell type).
Shipping and Storage:
FANA oligos are shipped in lyophilized form. Upon arrival store them in -20°C.
When ready to use, mix FANA oligos with sterile water or your favorite buffer. It is recommended to make multiple aliquots to avoid multiple freez thaw cycles.