Your RNA Sequencing Solutions!
RNA-Seq (RNA sequencing) is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample,providing a snapshot of gene expression in the sample, also known as transcriptome. Understanding the transcriptome is key if we are to connect the information in our genome with its functional protein expression. This allows scientists to understand the biology of a cell more deeply and assess changes that may indicate disease.
RNA Sequencing Workflow
Sciencewerke provides solutions to your RNA sequencing needs:
Purification & Isolation
Norgen Biotek Total RNA Purification Kits
- Extract high quality and purity total RNA, including miRNA, from a wide range of specimens including miRNA, from a wide range of specimens
- No phenol step required
- Bind & elute all RNA irrespective of size or GC content, without bias
- Efficiently extract small RNA irrespective of GC content
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing
Click here to view the video tutorial on total RNA purification (Cat. 17200). Learn how to use one of Norgen Biotek's Best-Selling-Kits, the Total RNA Purification Kit (Cat. 17200), for the rapid purification of total RNA.
Library Preparation
Lexogen RiboCop rRNA Depletion Kit
Total RNA is comprised of large amounts of undesired ribosomal RNA (rRNA). RiboCop enables very efficient removal of rRNA. Besides ribosomal RNA, RiboCop can also deplete globin mRNA, which makes up 30 – 80 % of all mRNA in mammalian blood. Thus, RiboCop focuses sequencing reads on RNA of interest while maintaining unbiased expression profiles.
RiboCop also offers exceptional performance on low input samples, down to 1 ng starting amount and is the ideal solution for total RNA-Seq analysis on degraded and FFPE samples.
Applications:
- Expression analysis, incl. non-coding transcripts
- Analysis of long non-coding RNA, snRNAs, snoRNAs, and circRNAs
- Isoform analysis, incl. non-polyadenylated RNAs
- FFPE and Biobank transcriptomics
- Biomarker analysis, e.g., circRNAs in blood, and many more…
Click here more information on Lexogen RiboCop rRNA Depletion Kit.
Library Preparation
Lexogen CORALL RNA-Seq Library Prep Kits
The CORALL RNA-Seq Library Prep Kits enables fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries for whole transcriptome analyses using Illumina® NGS platforms.
- Time saving: Offers a fast and fully automatable whole transcriptome RNA- Seq workflow which is completed in only 4.5 hours.
- Excels with low input: Supports the widest input range (1 ng – 1000 ng) with excellent performance for low input RNA down to 1 ng prior to mRNA- selection or ribo-depletion.
- Unlocks challenging samples: CORALL’s fragmentation-free protocol and built-in UMIs for RNA-Seq library preparation are ideal for challenging inputs, such as degraded and FFPE RNA.
- Adjustable library size to perfectly fit your application: Choose between standard size for expression analysis or longer lengths for alternative splicing, isoforms and fusion detection, etc.
Click here try the CORALL Configurator find the ideal kit for your application in less than 2 minutes.
Click here for more information about Lexogen CORALL RNA-Seq Library Prep Kits.