ClonaCell™ Semi-solid Cloning medium and Cell Line Development

The ClonaCell™-HY method uses a methylcellulose-based semi-solid selective medium to combine hybridoma selection and cloning into one step. Individual parental hybridoma clones and their progeny remain localized together in the semi-solid medium as they grow to form distinct colonies. This prevents the loss of rare clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium. The hybridoma colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion.