The whole cell lysates, heat shock pretreated, are manufactured using the cells and cell lines of the CLS portfolio. Each vial contains 100 µg protein in 200 µl RIPA buffer containing protease inhibidors. We recommend to apply 20 to 25 µg per lane. Storage: Short term at -20°C (1-2 days); long term at -70°C. Repeated freezing and thawing should be reduced to a minimum.
Applications:
– Positive control in Western Blottingpar
– Immunprecipitation
– Identification and screening of Biomarkers
– Detection and characterization of Antibodies
– Studies of Protein Expression and Interaction
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