Maurice innovates the conventional capillary electrophoresis technology to automate your protein profiling either by size or charge. Need cIEF and CE-SDS data for your mAbs, ADCs, vaccines or virus-like particles? Maurice does it all. Just pop in one of the preassembled cartridges, drop in your sample vials or a 96-well plate, and hit start. You will complete method development in a day; and get high-resolution, reproducible data in minutes.
Maurice affords high-resolution characterization of charge variants by preserving our proprietary imaged capillary isoelectric focusing (icIEF) technology, which has been the go-to method for measuring charge heterogeneity. The whole-column detection eliminates the post-focusing mobilization step needed with traditional cIEF, and can resolve your most challenging molecules including fusion proteins and hydrophobic ADCs.
| ATURE | cIEF SPECIFICATION | CE-SDS SPECIFICATION |
|---|---|---|
| Minimum Sample Volume | 50 µL | 50 µL |
| Sample Delivery | Vacuum | Electrokinetic |
| Typical Separation Time | 6–10 min (molecule-dependent) | Reduced IgG: 25 min, Non-reduced IgG: 35 min |
| Detection Capability | UV Absorbance at 280 nm Fluorescence: Ex 280 nm, Em 320–450 nm | UV Absorbance at 220 nm |
| Typical Voltage | Pre-focusing: 1500 V, focusing: 3000 V | Separation: 5750 V |
| Sample Injections per Cartridge | 100 guaranteed, 200 maximum | 100 guaranteed, 200 maximum |
| Maximum Sample Injections per Batch | 100 | 48 |
| pI/Size Range | 2.85–10.45 | 10–270 kDa |
| pI/Sizing CV | 1% | ≤2% |
| CV for Peaks >10% Composition | ≤5% (Intra-batch), ≤6% (Inter-batch) | N/A |
| Relative Migration Time CV | N/A | <1% for reduced IgG |
| pI/Sizing Resolution | 0.05 pI units (for wide range 3–10 ampholytes) | ≥1.5 for NGHC/HC IgG Standard |
| Dynamic Range | 2 logs | 2 logs |
| Linearity | >0.995 | >0.995 |
| Sensitivity (LOD) | 0.7 µg/mL (Native fluorescence) 3.0 µg/mL (Absorbance) (Values based on a monoclonal antibody) | 0.3 µg/mL (Value based on Internal Standard) |
| Sample Tray Options | 96-well plates or 48 vials | |
| Dimensions | 44cm H x 42cm W x 61cm D | |
| Weight | 46 kg (100 lb) | |