PEPperPRINT’s proprietary peptide laser printing technology, the high-density PEPperCHIP® Peptide Microarrays provides a platform for:
Antigen and Epitope Discovery
Epitope Mapping
Epitope Substitution Scans
Immune Monitoring
Serum Biomarker Discovery
Peptide Target Binder Development
Analysis
PEPperCHIP® Peptide Microarray analysis is straightforward and can be done in your own lab or by PEPperPRINT experts within a PEPperMAP® Service Offer. Further information about lab requirements for peptide microarray analysis can be found here.
Timeline
From peptide array design to production and quality control, it takes about 3 weeks.
Advantages
Highest spot densities and numbers of peptides per chip
Widely unlimited peptide content flexibility
Adjustment of peptide microarray layouts according to your specifications
Cost-effective solutions without excessive and expensive peptide pre-synthesis
Multiple peptide array copies per chip for one-by-one assays
Outstanding signal-to-noise ratios due to custom slide coatings
Straighforward handling and analysis
Comprehensive support before, during and after purchase
Specifications
From single antigens translated into overlapping peptides to whole virus proteomes – PEPperCHIP® Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner.
| Peptide Synthesis: | On-chip using Fmoc chemistry and routine double coupling steps |
| Microarray Format: | Standard slide format (3” x 1”, 75.4 mm x 25.0 mm x 1.0 mm) |
| Number of Peptides: | From 130 to 5,500 peptides printed in duplicate |
| Arrays Copies per Chip: | From single arrays to 16 arrays per chip depending on the number of peptides |
| Array Content: | Custom protein-derived overlapping peptides, peptide collections, substitution scans etc. |
| Available Amino Acids: | L- and D-amino acids, citrulline and other amino acids on request |
| Peptide Length: | Routine synthesis of up to 15mer peptides, longer peptides on request |
| Control Peptides: | HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) epitopes, other controls on request |
Input Data & Sample Material
10-20 µl serum/plasma or 10-20 µg purified antibody
Protein sequences (e.g. FASTA format or UniProt ID) or peptide collections (Excel, Word or text files)
Further Information
Application Notes
Publications
Protocols
Webinars and Movies
Testimonials