Principle
Platelia Rabies II Ad Usum Veterinarium is an immuno-enzymatic technique for the detection of rabies
virus anti-glycoprotein antibodies. This assay can be carried out on the serum of several species of
animals - dog, cat and fox. The test is based on the use of a solid phase enzyme immunoassay
technique referred to as an indirect ELISA. A microplate is coated with rabies glycoprotein
extracted from the inactivated and purified virus membrane. The enzymatic conjugate consists of a
protein A from Staphylococcus aureus coupled with peroxidase. Positive controls, calibrated against
OIE standard, allow the qualitative or quantitative determination of anti-rabies antibody titre in
the serum.
Features & Benefits
Simplicity of the ELISA techique
compared to virus neutralization
Rapidity: results available in
less then 3 hours
Manipulation safety: no handling
of live rabies virus
Highly reproducible results
Qualitative and quantitative
protocol
A microplate is coated with
rabies glycoprotein extracted from the inactivated and purified virus membrane
Used for Individual serology for
international trade purposes and Confirmation of the vaccination status
Kit Components
- Microplate (R1) ; 2 plates
- Wash solution (R2) ; 1 vial (250 ml)
- Negative control (R3) ; 1 vial (0.6 ml)
- 0.5 EU/ml Positive control (R4a) ; 1 vial (0.6 ml)
- 4 EU/ml Positive control (R4b) ; 1 vial (0.6 ml)
- Sample diluent (R6) ; 2 vials (2 x 125 ml)
- Conjugate (R7) ; 1 vial (3 ml)
- Peroxydase substrate buffer (R8) ; 1 vial (60 ml)
- Chromogen (R9) ; 1 vial (5 ml)
- Stop solution (R10) ; 1 vial (28 ml)
- Adhesive films for microplates ; 6
Product Size
Enough reagents are provided for 2 x 96 assays. On each microplate, 90 samples
can be analysed if a qualitative test is performed. Up to 80 samples can be quantified precisely for
rabies antibodies if a quantification system is used.
Storage
All the components are stored at 2° to 8°C and can be used until the expiry date
indicated on the kit. Reagent R2 (10 X) can be stored at 2° to 25°C before reconstitution.
User Guide
Safety Data Sheet
