QuantSeq 3′ mRNA-Seq Library Prep Kit
The QuantSeq FWD Kit is a library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
The QuantSeq FWD Kit is a library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
The QuantSeq FWD Kit is a library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
QuantSeq FWD contains the Illumina Read 1 linker sequence in the second strand synthesis primer, hence NGS reads are generated towards the poly(A) tail, directly reflecting the mRNA sequence (see workflow). This version is the recommended standard for gene expression analysis. With the QuantSeq FWD HT Kit (015.384) Lexogen furthermore provides a high-throughput version with optional dual indexing (i5 and i7 indices) allowing up to 384 samples to be multiplexed in one lane.
Cost-saving and streamlined globin mRNA depletion during QuantSeq library preparation
Genome-wide analysis of gene expression
Cost-efficient alternative to microarrays and standard RNA-Seq
Down to 100 pg total RNA input
Applicable for low quality and FFPE samples
Free data analysis pipeline on the Bluebee® genomics analysis platform
Single-read sequencing of up to 9,216 samples/lane
Dual indexing and Unique Molecular Identifiers (UMIs) are available