QX200 Droplet Digital PCR System
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Bio-Rad’s QX200 ddPCR System combines water-oil emulsion droplet technology with microfluidics. The QX200 System consists of two instruments — a droplet generator and a droplet reader --and associated consumables. The droplet generator partitions each sample into 20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are distributed in a random fashion. Each droplet serves to partition the reactions. Droplets are transferred to a 96-well PCR plate and PCR is performed to end point in a thermal cycler. The droplets stream single file through the reader for fluorescence analysis. Positive droplets, which contain at least one copy of the target DNA or RNA molecule, exhibit increased fluorescence compared to negative droplets. The fraction of PCR-positive droplets enables the target to be quantified according to the Poisson distribution.