Oligos designed using next generation FANA technology can effectively and efficiently perform:
Efficient knock down or regulation of the target RNA.
Ability to bind to the target RNA (mRNA, miRNA or lncRNA) in a high sequence specific manner.
No toxicity.
Efficient delivery without an external source (e.g. without a transfection agent, formulation, conjugate or viral vector).
Viral RNA Silencing
AUMsilence
Key features: Superior alternative for other siRNA, shRNA and CRISPR. No transfection reagents required. No toxicity
AUMsilence self delivering oligos achieve highly efficient and potent antisense-based viral RNA knockdown. No need to use toxic transfection reagents. Especially designed for primary cells and very difficult to transfect cell types. Ideal for animal studies. Suited for fish and amphibian model systems as well.Highly recommended as a replacement for siRNAs, shRNA, CRISPR or other oligo approaches.We take pride in having the simplest protocol for RNA silencing studies. FANA oligos can work with any cell type without causing toxicity. FANA can work with easy to transfect, hard to transfect (like primary cells, B-Cells, T-Cells, neurons etc.) and highly sensitive patient samples.
Protocol:
(1) Depending upon the experiment different time points can be used to measure knock down or related effects for up to several days using a single dose (and weeks in some cases).
(2) In certain cases (especially for very fast growing cells) if the knock down effect is reduced after a few days, simply add more FANA oligos to the cell culture.
(3) FANA oligos can be fluorescently labeled (or with any desired label) to monitor cellular uptake or biodistribution.
Depending upon the experiment different time points can be used to measure knock down or related effects for up to several days using a single dose (and weeks in some cases).
In certain cases (especially for very fast growing cells) if the knock down effect is reduced after a few days, simply add more FANA oligos to the cell culture.
FANA oligos can be fluorescently labeled (or with any desired label) to monitor cellular uptake or biodistribution.
Still want to use transfection reagents: If, for any reason, you still prefer to use transfection reagents the recommended working concentration is from 1nM to 25 nM (depending upon the cell type).
Shipping and Storage:
FANA oligos are shipped in lyophilized form. Upon arrival store them in -20°C.
When ready to use, mix FANA oligos with sterile water or your favorite buffer. It is recommended to make multiple aliquots to avoid multiple freez thaw cycles.